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Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
《工程(英文)》 doi: 10.1016/j.eng.2023.02.016
关键词: Glycoproteins N-glycans Reversible labeling Hydrophilic interaction liquid chromatography Capillary gel electrophoresis Mass spectrometry
王彦吉,赵鹏程,徐媛媛,王景翰
《中国工程科学》 2007年 第9卷 第2期 页码 25-29
讨论了优化电泳条件建立区带毛细管电泳法分析黑色签字笔字迹墨水的分析方法,采用20mmol/L四硼酸钠,pH8.5,未涂层熔融石英毛细管内径100μm,有效柱长40cm,15kV分离电压,光二极管阵列检测器 (PDA),检测波长190~600nm的电泳分离条件,对不同种类黑色签字笔字迹墨水提取液进行了分析;利用毛细管电泳PDA检测器在可见光波长下检测出的黑色签字墨水中不同染料相对含量的变化关系,研究了字迹相对形成时间问题,得出较好的变化规律。
可去除染料——N-聚糖多方法深入分析中的缺失环节 Article
Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
《工程(英文)》 2023年 第26卷 第7期 页码 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016
As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans' immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach "visualizes" N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.
Application of proteomics in environmental science
Xiaona CHU, Jiangyong HU, Say Leong ONG,
《环境科学与工程前沿(英文)》 2009年 第3卷 第4期 页码 393-403 doi: 10.1007/s11783-009-0145-x
关键词: proteomics environmental stress two dimensional (2D) gel electrophoresis mass spectrometry
Current molecular biologic techniques for characterizing environmental microbial community
Dawen GAO, Yu TAO
《环境科学与工程前沿(英文)》 2012年 第6卷 第1期 页码 82-97 doi: 10.1007/s11783-011-0306-6
关键词: molecular biological technique microbial community denaturing gradient gel electrophoresis (DGGE) terminal restriction fragment length polymorphism (T-RFLP) environmental applications
null
《医学前沿(英文)》 2018年 第12卷 第2期 页码 196-205 doi: 10.1007/s11684-017-0560-5
We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay (RTCA), and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% (81/399). The mPCR-CE had a specificity of 97.2% and a sensitivity of 96.0%, which was higher than RTCA (x2 = 5.67, P = 0.017) but lower than BD MAX Cdiff (P = 0.245). Among the 45 strains, 44 (97.8%) were determined as non-ribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 (n = 8, 17.8%), 001 (n = 6, 13.3%), and 012 (n = 7, 15.6%) were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.
关键词: Clostridium difficile multiplex PCR capillary electrophoresis detection characterization
Seasonal microbial community shift in a saline sewage treatment plant
Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG
《环境科学与工程前沿(英文)》 2011年 第5卷 第1期 页码 40-47 doi: 10.1007/s11783-011-0304-8
关键词: sewage treatment plants polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) canonical correspondence analysis correspondence analysis
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
《环境科学与工程前沿(英文)》 2011年 第5卷 第2期 页码 175-185 doi: 10.1007/s11783-011-0320-8
关键词: quantitative polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) principal component analysis (PCA) canonical correspondence analysis (CCA)
null
《医学前沿(英文)》 2012年 第6卷 第4期 页码 366-375 doi: 10.1007/s11684-012-0214-6
Surveillance is critical for the prevention and control of infectious disease. China’s real-time web-based infectious disease reporting system is a distinguished achievement. However, many aspects of the current China Infectious Disease Surveillance System do not yet meet the demand for timely outbreak detection and identification of emerging infectious disease. PulseNet, the national molecular typing network for foodborne disease surveillance was first established by the Centers for Disease Control and Prevention of the United States in 1995 and has proven valuable in the early detection of outbreaks and tracing the pathogen source. Since 2001, the China CDC laboratory for bacterial pathogen analysis has been a member of the PulseNet International family; and has been adapting the idea and methodology of PulseNet to develop a model for a future national laboratory-based surveillance system for all bacterial infectious disease. We summarized the development progress for the PulseNet China system and discussed it as a model for the future of China’s national laboratory-based surveillance system.
关键词: infectious disease laboratory-based infectious disease surveillance pulse field gel electrophoresis multilocus sequencing typing PulseNet China
Simulation on refrigerant flow in adiabatic capillary tube
WANG Meixia, LIU Cunfang, ZHOU Qiangtai
《机械工程前沿(英文)》 2008年 第3卷 第3期 页码 332-336 doi: 10.1007/s11465-008-0054-x
关键词: mathematical diameter literature sub-cooling adiabatic capillary
Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE
《环境科学与工程前沿(英文)》 2015年 第9卷 第2期 页码 324-333 doi: 10.1007/s11783-014-0627-3
关键词: aerobic granular sludge completely mixed activated sludge system (CMAS) continuous flow shear force filamentous bacteria polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)
Xuhong Zhang, Haimiao Li, Xin Zhang, Meng An, Weiwei Fang, Haitao Yu
《化学科学与工程前沿(英文)》 2017年 第11卷 第2期 页码 231-237 doi: 10.1007/s11705-017-1633-3
关键词: gelator Schiff base chiral recognition gel formation gel collapse
Elena CERRO-PRADA,Miguel MANSO,Vicente TORRES,Jesús SORIANO
《结构与土木工程前沿(英文)》 2016年 第10卷 第2期 页码 189-197 doi: 10.1007/s11709-015-0326-6
关键词: cement composites photocatalytic TiO2 sol-gel C-S-H gel microstructure
Mark BOMBERG
《结构与土木工程前沿(英文)》 2010年 第4卷 第4期 页码 431-437 doi: 10.1007/s11709-010-0071-9
关键词: capillary active insulation integrated heating cooling and ventilation air conditioning (HVAC) and building enclosure dynamic insulation switchable thermal resistance variable U-value walls
HE Maogang, ZHANG Ying, SONG Xinzhou, ZHANG Jiantao
《能源前沿(英文)》 2008年 第2卷 第1期 页码 36-42 doi: 10.1007/s11708-008-0013-6
标题 作者 时间 类型 操作
Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
期刊论文
Current molecular biologic techniques for characterizing environmental microbial community
Dawen GAO, Yu TAO
期刊论文
Simultaneous detection and characterization of toxigenic:Clostridium difficile directly from clinical stool specimens
null
期刊论文
Seasonal microbial community shift in a saline sewage treatment plant
Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG
期刊论文
Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium
Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO
期刊论文
PulseNet China, a model for future laboratory-based bacterial infectious disease surveillance in China
null
期刊论文
Simulation on refrigerant flow in adiabatic capillary tube
WANG Meixia, LIU Cunfang, ZHOU Qiangtai
期刊论文
Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system
Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE
期刊论文
Visual chiral recognition of 1,1′-binaphthol through enantioselective collapse of gel based on an amphiphilic
Xuhong Zhang, Haimiao Li, Xin Zhang, Meng An, Weiwei Fang, Haitao Yu
期刊论文
Microstructural and photocatalytic characterization of cement-paste sol-gel synthesized titanium dioxide
Elena CERRO-PRADA,Miguel MANSO,Vicente TORRES,Jesús SORIANO
期刊论文
A concept of capillary active, dynamic insulation integrated with heating, cooling and ventilation, air
Mark BOMBERG
期刊论文